30 LABORATORY GUIDE IN BACTERIOLOGY 



The weight of the mixture should be 1,000 grams. 

 Tube hot, without filtration, through a pipette with a 

 wide aperture. Autoclave for five minutes at 120 C., 

 and allow to cool in a slanting position. 



EXERCISE 7. STANDARD METHOD OF PREPARING BROTH, 



NUTRIENT GELATIN, AND NUTRIENT AGAR 



STANDARD METHODS FOR THE EXAMINATION OP WATER AND 



SEWAGE. AMERICAN PUBLIC HEALTH ASSOCIATION 



755 BOYLSTON STREET, BOSTON, MASS. 



9. Titrate after boiling one minute to expel carbon dioxid. 



10. Adjust reaction to i% acid by adding normal hydrochloric acid or sodium 

 hydrate as required. 



11. Heat over boiling water (or steam) bath for 40 minutes. 

 la. Restore loss by 



g water (or s 



evaporation. 



