TABLE OF CONTENTS 



PART I. BACTERIOLOGICAL TECHNIC 



Section i. Laboratory Rules 



Section 2. General Directions 



Preparation and Cleaning of Glassware 

 Methods of Sterilization .... 



Preparation of Culture Media . . 



Preparation of Staining Solutions 



The Microscope ....... 45 



Scheme for Routine Study of Bacteria . 50 

 Method of Describing Cultures ... 53 

 Directions for Filling out Culture Charts 58 



Section 

 Section 

 Section 

 Section 

 Section 

 Section 

 Section 9. 

 Section 10. 



PAGE 



3 



4 



8 



10 



18 



43 



PART II. GENERAL BACTERIOLOGY 



Section i. Preparation of Culture Media ... 69 

 Section 2. Collecting and Cultivating Micro-organ- 

 isms from the Air 70 



Section 3. Study of Molds, Yeasts, and Torulae . 78 

 Section 4. Bacteriological Examination of Water, 



Air, and Milk . 84 



Section 5. Exercises on Infection and Sterilization . 91 

 Section 6. Influence of Disinfectants, Light, and 



Heat on the Growth of Micro-organisms 94 



Section 7. Study of Chromogenic Bacteria ... 97 



Section 8. Study of Micrococci 100 



Section 9. Study of Intestinal Bacteria . . . . 101 



PART III. IMPORTANT PATHOGENIC BACTERIA 



Section i. Preparation of Culture Media . . . 105 



Section 2. The Pyogenic Group 105 



Section 3. The Group of Colon-Typhoid Bacilli . 112 



Section 4. The Proteus Group . . .. . . . 121 



Section 5. The Capsulated Group 123 



Section 6. The Diphtheria Group 124 



