34 LABORATORY GUIDE IN BACTERIOLOGY 



Drigalski and Conradi's medium (modified by 

 Harris) 



Dextrose-free broth 2,000 c.c. 



Nutrose 20 grams 



Agar 40 grams 



Boil, dissolve, neutralize to phenolphthalem, auto- 

 clave at 120 C. for 5 minutes. Clarify with whites of 

 four eggs (or powdered egg albumin), and filter. Then 

 add after dissolving separately in water: 



Lactose 30 grams 



Litmus solution (see p. 35} 260 c.c. 



Crystal violet (o.i per cent solution) 20 c.c. 



Tube and sterilize in the arnold. 

 Parietti's solution 



Carbolic acid 5 c.c. 



Hydrochloric acid 4 c.c. 



Water 100 c.c. 



Phenol media. One part carbolic acid (phenol) is 

 added to 1,000 parts medium. 



Hiss's plating medium for colon-typhoid differ- 

 entiation 



Agar 15 grams 



Gelatin 15 grams 



Extract of meat 5 grams 



NaCl 5 grams 



Dextrose 10 grams 



Distilled water 1,000 c.c. 



Hiss's tube medium 



Agar 5 grams 



Gelatin 80 grams 



Extract of meat 5 grams 



NaCl 5 grams 



Dextrose 10 grams 



Distilled water 1,000 c.c. 



