BACTERIOLOGICAL TECHNIC 39 



Solution for testing the formation of nitrates 



MgSO 4 0.3 gram 



FeS0 4 0.4 gram 



NaCl 0.5 gram 



K 2 HPO 4 0.5 gram 



Fused Na 2 CO 3 i gram 



NaNO 2 i gram 



Distilled water 1,000 c.c. 



Dissolve 20 grams agar in the above solution. 

 Sterilize in the autoclave. The salt solution may also 

 be added to the silica jelly. For plating fill in culture 

 tubes. 



Solution for testing the assimilation of atmospheric 

 nitrogen 



i. Solution 



KH 2 PO 4 i gram 



MgSO 4 o. 50 gram 



NaCl o . 01 gram 



FeSO 4 o . 01 gram 



MnSO 4 o . 01 gram 



Dextrose 20 grams 



Distilled water 1,000 c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



2. Mannit Solution 



K 2 HPO 4 o . 20 gram 



CaCl 2 0.02 gram 



MgSO 4 o . 20 gram 



Fe 2 Cl6 o . 01 gram 



Mannit 15 grams 



NaOH solution until neutral to phenolphthalem 

 Distilled water 1,000 c.c. 



Dissolve 20 grams agar in this solution if intended 

 for plating and tubing. Sterilize in the autoclave. 



