44 LABORATORY GUIDE IN BACTERIOLOGY 



water. After solution add enough distilled water to 

 bring the volume to 300 c.c. 



Other useful stains are the following: 



Delafield's hematoxylin 



Hematoxylin crystals 4 grams 



Alcohol 25 c.c. 



Ammonia alum 50 grams 



Water 400 c.c. 



Glycerin 100 c.c. 



Methyl alcohol 100 c.c. 



Alum hematoxylin 



1. Hematoxylin 2 grams 



Absolute alcohol 100 c.c. 



2. Ammonia alum 2 grams 



Water 100 c.c. 



Mix i and 2 and add 



Glycerin 850 c.c. 



Glacial acetic acid 100 c.c. 



Allow to stand for one month before using. 



Bismarck brown 



Bismarck brown 0.5 gram 



Water ; 100 c.c. 



Safranin 



Safranin 0.5 gram 



Water 100 c.c. 



Carbolic thionin blue (Nicolle) 



Thionin blue i gram 



Carbolic acid 2.5 grams 



Water 100 c.c. 



Alum carmin 



Alum 2.5 grams 



Carmin i . o gram 



Water . . .100 c.c. 



