FROST'S CULTURE CHART (MODIFIED) 



Group 



Name of organism. 



Source 



Habitat 



References 



PHYSIOLOGICAL CHARACTERS 



1 Relation to temperature: (a) Optimum. . . (b) Minimum. . . (c) Maximum. 



2 Relation to free oxygen: 



Relation to other agents such as: 



desiccation, light, disinfectants, etc: 



4 Pigment production: 



5 Growth in carbohydrate media: 



a. Stab or shake culture (dextrose agar): gas production 



6. Fermentation tube: (i) growth in bulb:. . (2) growth in closed arm: 



After 24 hours 

 After 48 hours . 



Gas formula ~j 



d. Reaction (i) in bulb (2) in closed arm. 



6. Acid or alkali production (in litmus milk) 



7. Reduction of nitrates to nitrites to ammonia 



8. Indol production; 24 hours 48 hours days 



fecal odor; 24 hours 48 hours days. ....... 



9. Enzym production; (a) proteolytic (b) coagulative. . . . (c) diastatic. . 



10. Characteristic odor 



11. Agglutination 



12. Pathogenesia 



