SECTION i 

 PREPARATION OF CULTURE MEDIA 



The following culture media are to be prepared for 

 this work : 



FIRST SET 



Wort agar 20 tubes 



Ten of these tubes should contain about 7 c.c. for slants, the 

 other ten about 10 c.c. for plating. 



Wort gelatin ' 10 tubes 



Liquid wort 10 tubes 



Fermentation tubes 6 tubes 



Two of the fermentation tubes to contain i per cent dextrose, 

 two i per cent lactose, and the remaining two i per cent saccha- 

 rose. 



SECOND SET 



Meat extract agar 20 tubes 



Ten of these for slants and ten for plating. 



Pepton gelatin 15 tubes 



Broth 15 tubes 



Dextrose agar. 15 tubes 



Litmus milk 15 tubes 



Synthetic potato 10 tubes 



The beerwort media are to be prepared during the 

 nrst week, the other media later. 



