IMPORTANT PATHOGENIC BACTERIA 131 



g) Transfer a loopful from the surface of the second 

 flask to the third one, and incubate as before. 



h) After 18-24 hours, again stain and examine for 

 spirilla. By this time a film has formed which contains 

 the spirilla practically in pure culture. 



5. Special study. Inoculate a pigeon intramuscu 

 larly with 0.5 c.c. of a broth culture of Sp. metch- 

 nikovii. The breast of the pigeon is laid bare, washed 

 with mercuric chlorid and alcohol, and the syringe 

 plunged into the muscle fibers and discharged. After 

 death, note the peculiar appearance, resembling that 

 of boiled beef. Make stained preparations from the 

 blood and muscle juice, and examine for spirilla. 



SECTION 10 

 THE GROUP OF ACID-PROOF BACILLI 



Members 



Bacillus tuberculosis. 



B. leprae. 



B. smegmae. 



Moller's grass bacillus, and a number of bacilli found on 

 grass, dung, in butter, milk, etc. 



Cultural studies of B. tuberculosis consume a great 

 deal of time and are, therefore, impracticable in an 

 elementary course. 



For comparison, the culture characteristics of 

 Moller's grass bacillus are instructive. 



Inoculate an agar slant from a laboratory culture of 

 Moller's grass bacillus. 



i. Routine study. 



