MOLDS, YEASTS, TORULAE, AND BACTERIA 183 



6. If the spores are numerous dilute the suspension 

 until only a few appear in a loopful. 



7. When the dilution is high enough to show but 

 a few spores under the microscope transfer a large 

 loopful to a cover slip. 



: Ji> 



FIG. 34 



Sterilizing Wort in a Cask 



a. Cask b. Rubber stopper c. Cotton plugs d. Test tube 

 e. Glass tubes /. Cotton stopper and connection with autoclave 



8. Invert this cover slip over the ring of a Bb'ttcher 

 Moist Chamber (Fig. 35). 



9. Incubate at 37 C. 



10. Observe the hanging drop every day and make 

 sketches. After 7 to 10 days the cycle of development 

 will be completed so that a new crop of spores has 

 appeared. 



