THE TRYPANOSOMES 41 



a. Outer clear portion, the ectosarc. 



b. Inner granulated portion, the entosarc. 



c. The irregular projections, the pseudopodia, in which the 

 ectosarc is best seen. These are organs of locomotion. 



d. In the entosarc a dark globular body is visible, the nucleus. 



e. Some specimens may show one or more clear globular 

 bodies in the entosarc, the vacuoles. 



Draw several specimens. 



B. Examine the slide for encysted amoebae. 



EXERCISE 23b 



THE TRYPANOSOMES 



PHYLUM PROTOZOA, CLASS FLAGELLATA (MASTIGOPHORA), ORDER 

 LISSOFLAGELLATA 



A. In prepared samples of blood smears from an animal af- 

 fected with Nagana, note the presence of spindle-shaped organ- 

 isms with a whip-like appendage, Trypanosoma brucei, the try- 

 panosome of Nagana. 



a. A single whip-like appendage, the flagellum. 



b. A thin fold along the dorsal edge, the undulating membrane. 



c. Near the middle of the body a dark object, the nucleus. 



d. Near the anterior end (the flagellum end) of the body, a 

 small mass of chroma tin, the blepharoplast. 



Draw several specimens. 



B. In a suitable blood smear from a rat, examine for try- 

 panosomes, in this case Trypanosoma lewisi. 



Compare A and B. 



C. In a blood smear taken from a patient suffering from Afri- 

 can sleeping sickness, first stage, examine for Trypanosoma gam- 

 biense. 



Compare this with A and B. 



