SOIL EXTRACT (FLAGELLATES AND CILIATES) 97 



should be between (+0.8 or +1.0). It requires about 

 10 c.c. of N/i NaOH per liter. In place of clarifying with 

 the white of egg, the author recommends heating for half 

 an hour at 15 pounds' pressure without disturbing the sedi- 

 ment and decanting through a cotton filter. 



Conn, H. J., Bui. 38, New York Agr. Exp. Sta., 1914. 



COUNTING SOIL PROTOZOA 



Medium n 

 Hay-soil Extract 



Soil extract 100 c.c. 



Hay extract (o.i per cent, of dry hay) 100 c.c. 



Calcium carbonate (CaCO 3 ) 5 gm. 



Tap-water 800 c.c. 



Medium 12 

 Hay Infusion 



Hay 10 gm. 



Tap-water 1000 c.c. 



Medium 13 

 Hay Egg-albumen (Ciliates) 



Hay 100 gm. 



Egg-albumen 50 gm. 



Tap-water 1000 c.c. 



Medium 14 

 Soil Extract (Flagellates and Ciliates) 



Soil extract 100.0 c.c. 



Dibasic potassium phosphate (K^HPC^) 0.5 gm. 



Tap-water 900.0 c.c. 



7 



