98 SOIL BACTERIOLOGY 



Medium 15 

 Mannit Solution 

 See Culture-medium No. 39, page 108. 



AMMONIFICATION 



Medium 16 



Peptone Solution 



Peptone 10 gm. 



Distilled water 1000 c.c. 



Heat in the autoclave for thirty minutes and filter. 



Medium 17 

 Gelatin Solution 



Gelatin 5 gm. 



Distilled water. 1000 c.c. 



Bring the reaction to (+1.0). 



Medium 18 



Casein Solution 



Casein 10 gm. 



Normal sodium hydroxid (NaOH) 7 c.c. 



Distilled water 1000 c.c. 



Prepare according to directions on page 94. 



Medium 19 

 Urea Solution 



Urea (CO(NH 2 ) 2 ) 20 gm. 



Bouillon ( i.o) looo c.c. 



