SOLUTION FOR NITRITE 



Medium 27 

 Solution for Nitrite Formation 



Magnesium ammonium phosphate (Mg(NH4)PO4 



+ 6H 2 O) 2.0 gm. 



Dibasic potassium phosphate (KaHPO^ 0.5 gm. 



Magnesium sulphate (MgSO 4 + 7H2O) 0.5 gm. 



Sodium chlorid (NaCl) i.o gm. 



Ferrous sulphate (FeSO 4 + 7H 2 O) 0.4 gm. 



Magnesium carbonate (MgCO 3 ) 5- gm- 



Distilled water 1000.0 c.c. 



It is not necessary to sterilize the magnesium ammonium 

 phosphate separately. 



Medium 28 

 Solution for Nitrate Formation 



Sodium nitrite (NaNO2) i.o gm. 



Dibasic potassium phosphate (K 2 HPO4) 0.5 gm. 



Magnesium sulphate (MgSC>4 + 7H 2 O) 0.3 gm. 



Sodium chlorid (NaCl) 0.5 gm. 



Ferrous sulphate (FeSO4 + yH 2 O) 0.4 gm. 



Sodium carbonate (Na 2 CO 3 ) (anhydrous) 0.3 gm. 



Distilled water : IOOG.O c.c. 



The formation of nitrates takes place rapidly, provided 

 the cultures are grown under conditions that supply an 

 abundance of oxygen. 



Medium 29 

 Solution for Nitrite and Nitrate Formation 



Ammonium sulphate (NH 4 )2SO 4 2.0 gm. 



Dibasic potassium phosphate (K 2 HPC>4) i.o gm. 



Magnesium sulphate (MgSC>4 + ?H 2 O) 0.5 gm. 



Sodium chlorid (NaCl) 2.0 gm. 



Ferrous sulphate (FeSO4 + yH 2 O) 0.4 gm. 



Calcium carbonate (CaCOs) 5.0 gm. 



Distilled water. . . 1000.0 c.c. 



