MANNIT SOLUTION IOQ 



Medium 40 



__ 



Dextrose Solution 



Dibasic potassium phosphate (K^HPCU) 0.2 gm. 



Dextrose (CeHiaOe) 10.0 gm. 



Tap-water 1000.0 c.c. * 



For a solid medium add 1.5 per cent, of agar. 



(Anaerobic) 



Medium 41 

 Solution for Anaerobic Organisms 



Dibasic potassium phosphate (K 2 HPO 4 ) i.o gm. 



Magnesium sulphate (MgSO 4 + 7H 2 O) 0.2 gm. 



Sodium chlorid (NaCl) o.oi gm. 



Ferrous sulphate (FeSO4 + yH^O) o.oi gm. 



Manganese sulphate (MnSO 4 + 4H 2 O) o.oi gm. 



Dextrose (CeEfoQu) 20.0 gm. 



Calcium carbonate (CaCO 3 ) 30.0 gm. 



Distilled water 1000.0 c.c. 



Sterilize at 15 pounds' pressure for fifteen minutes; or, bet- 

 ter, steam twenty minutes for three consecutive days. 



Winogradsky, S., Centbl. Bakt. (etc.), Abt. 2, Bd. 9, p. 49, 1902. 



B. Symbiotic Nitrogen-fixing Bacteria 



Medium 42 

 Mannit Solution 



Mannit solution same as No. 39 with an excess of cal- 

 cium carbonate removed. 



/'I 



