HO SOIL BACTERIOLOGY 



Medium 43 

 Soil Extract 



Soil extract. 100.0 c.c. 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.5 gm. 



Mannit (C6H 8 (OH) 6 ) 10.0 gm. 



Distilled water 900.0 c.c. 



See note, page 95. 



Medium 44 



Saccharose Solution 



Saccharose (CuHaOu) 10.0 gm. 



Monobasic potassium phosphate (KH 2 PO 4 ) i.o gm. 



Magnesium sulphate (MgSO 4 + 7H 2 O) 0.2 gm. 



Distilled water 1000.0 c.c. 



Medium 45 

 Maltose Solution 



Maltose (CiaHaOii + H 2 O) 10.0 gm. 



Monobasic potassium phosphate (KH 2 PO 4 ) i.o gm. 



Magnesium sulphate (MgSO 4 + yH 2 O) o.i gm. 



Sodium chlorid (NaCl) : trace 



Ferrous sulphate (FeSO 4 + 7H 2 O) trace 



Calcium chlorid (CaCl 2 ) fused trace 



Distilled water 1000.0 c.c. 



In order to prepare a solid medium, add 1 5 grams of agar to 

 each liter of the above solutions. 



Medium 46 

 Bean-extract Caffein Agar 



Agar 15 gm. 



Caffein (C 8 H 10 N 4 O 2 + H 2 O) 2 gm. 



Dextrose (C 6 Hi 2 O 6 ) 20 gm. 



Bean extract. . . 1000 c.c. 



