SOLUTION FOR CELLULOSE FERMENTATION III 



Note. Bean extract: Add to 100 grams of powdered bean seed in a 

 mortar 100 c.c. of N/i KOH. Allow this to stand a few minutes, then add 

 water sufficient to make 5 liters. This should stand twenty-four hours. 

 Siphon off the clear liquid, neutralize with phosphoric acid (H 3 PO 4 + aq.), 

 and make the volume up to 5 liters. 



Zipfel, H., Centbl. Bakt. (etc.), Abt. 2, Bd. 32, pp. 107-131, 1911. 



Medium 47 

 Peptone Saccharose Solution 



Monobasic potassium phosphate (KH 2 PO 4 ) 2.0 gm. 



Magnesium sulphate (MgSO 4 + yEkO) o.i gm. 



Peptone i.o gm. 



Saccharose (Ci 2 H22On) 20.0 gm. 



Distilled water 1000.0 c.c. 



Buchanan, R. E., Centbl. Bakt. (etc.), Abt. 2, Bd. 22, p. 392, 1909. 



CELLULOSE DESTROYING ORGANISMS 



(Anaerobic) 



Medium 48 

 Solution for Cellulose Fermentation 



Dibasic potassium phosphate (K2HPO 4 ) i.o gm. 



Ammonium sulphate ((NH 4 )2SO 4 ) i.o gm. 



Magnesium sulphate (MgSO 4 + 7H 2 O) 0.5 gm. 



Calcium carbonate (CaCO 3 ) 2.0 gm. 



Sodium chlorid (NaCl) trace 



Distilled water 1000.0 c.c. 



Fill large test-tubes about half -full. Add strips of filter- 

 paper. 



Omelianski, W., Centbl. Bakt. (etc.), Abt. 2, Bd. 8, p. 226, 1902. 



