112 SOIL BACTERIOLOGY 



(Aerobic) 



Medium 49 



Solution for Cellulose Fermentation 



Dibasic potassium phosphate (K 2 HPO 4 ) ......... 0.5 gm. 



Ammonium chlorid (NH^Cl) ................... i.o gm. 



Calcium carbonate (CaCO 3 ) .................... 10.0 gm. 



Tap-water ................................... 1000.0 c.c. 



Prepare in shallow layers (loo-c.c. portions in 750-0.0. 

 Erlenmeyer flasks) and add one sheet of filter-paper about 

 10 cm. in diameter to each culture. 



Iterson, C. V., Centbl. Bakt. (etc.), Abt. 2, Bd. n, p. 693, 1904. 



Medium 50 

 Solution for Cellulose Fermentation 



Dibasic potassium phosphate (KaHPC^) ........... i gm. 



Magnesium sulphate (MgSC>4 + 7H2O) ............ i gm. 



Sodium carbonate (Na 2 CO 3 ), anhydrous ........... i gm. 



Ammonium sulphate ((NH^SC^) ....... . ........ 2 gm. 



Calcium carbonate (CaCO 3 ) ...................... 2 gm. 



Tap-water ...................................... 1000 c.c. 



Fill large test-tubes about half-full of the above medium, 

 or put i5o-c.c. portions into 300-0.0. Erlenmeyer flasks. 

 Immerse in the liquid of the test-tubes one or two strips of 

 filter-paper. In the Erlenmeyer flasks use a single sheet 

 of filter-paper of such a size that when dropped into the 

 liquid it will nearly cover the bottom of the flask. 



McBeth and Scales, Bui. 266, United States Dept. Agr. Bur. Plant 

 Indus., p. 26, 1913. 



