CELLULOSE AGAR 113 



Medium 51 

 Solution for Cellulose-fermenting Molds 



Rye bread or bran 10.0 gm. 



Dibasic potassium phosphate (K 2 HPO4) 0.5 gm. 



Dextrose (C 6 Hi 2 O 6 ) 2.0 gm. 



Distilled water 1000.0 c.c. 



The rye bread or bran and water are boiled together for 

 thirty minutes. The mixture is filtered, the loss in weight 

 restored, and the phosphate and dextrose added. 



Medium 52 

 Cellulose Agar 



Agar (washed) 15.0 gm. 



Cellulose 2.5 gm. 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.2 gm. 



Magnesium sulphate (MgSC>4 + 7H 2 O) 0.2 gm. 



Potassium carbonate (K^COs) 0.4 gm. 



Calcium chlorid (CaCl 2 ) fused 0.02 gm. 



Ferric sulphate (Fe 2 (SO 4 )3) 0.02 gm. 



Sodium chlorid (NaCl) 0.02 gm. 



Peptone 0.50 gm. 



Distilled water 1000.0 c.c. 



Dissolve the peptone in 40 c.c. of distilled water and filter. 

 Dissolve the salts in the filtrate and make volume equal to 

 100 c.c. Add 400 c.c. of the aqueous cellulose suspension 

 and 3 per cent, of aqueous washed agar. Tube in i5-c.c. 

 portions. 



Note. Hydra ted cellulose may be prepared as follows: To 100 c.c. of 

 concentrated sulphuric acid in a 2-liter flask add 60 c.c. of water. When 

 cooled to 60 C. add 5 grams of moist filter-paper. Shake this mass violently 

 until the cellulose is dissolved. Now fill the flask with water containing 



