Il6 SOIL BACTERIOLOGY 



SULPHUR ORGANISMS 



Reduction and Oxidation 



Medium 55 

 Solution for Sulphate Reduction 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.5 gm. 



Magnesium sulphate (MgSO 4 + 7H2O) i.o gm. 



Ferrous sulphate (FeSO 4 + 7H 2 O) trace 



Asparagin (C 4 H 8 N 2 O 3 + H 2 O) i.o gm. 



Sodium lactate (NaCsHsOa) 5.0 gm. 



Tap-water 1000.0 c.c. 



Van Delden, Centbl. Bakt. (etc.), Abt. 2, Bd. n, p. 88, 1904. 



Medium 56 

 Gelatin for Sulphate Reduction 



Gelatin 120.0 to 150.0 gm. 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.5 gm. 



Magnesium sulphate (MgSO 4 + yH 2 O) i.o gm. 



Iron-ammonium sulphate (FeSO 4 (NH 4 ) 2 (SO 4 ) + 



6H 2 O) trace 



Asparagin (C 4 HsN 2 O3 + H 2 O) i.o gm. 



Sodium lactate (NaCsHaOs) 5.0 gm. 



Distilled water- 1000.0 c.c. 



Sterilize in the autoclave at 10 pounds' pressure for fifteen 

 minutes. Cool in ice-water. 



Medium 57 

 Solution for Sulphate Reduction 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.5 gm. 



Sodium lactate (NaCaHsOs) 5.0 gm. 



Ammonium sulphate ((NH 4 ) 2 SO 4 ) 2.0 gm. 



Ferrous sulphate (FeSO 4 + 7H 2 O) trace 



Tap-water 1000.0 c.c. 



