Il8 SOIL BACTERIOLOGY 



Medium 60 

 Solution for the Oxidation of Thio sulphates 



Sodium thiosulphate (Na 2 S 2 O 3 + sH 2 O) 5.0 gm. 



Sodium hydrogen carbonate (NaHCO 3 ) i.o gm. 



Dibasic potassium phosphate (K 2 HPO 4 ) 0.2 gm. 



Ammonium chlorid (NEUCl) o.i gm. 



Magnesium chlorid (MgCl 2 ) + 6H 2 O) o.i gm. 



Tap-water 1000.0 c.c. 



Nathansohn, Mitt. a. d. zoolog. Station Neapel, Bd. 15, p. 655, 1902. 

 Beijerinck, W. M., Centbl. Bakt. (etc.), Abt. 2, Bd. n, pp. 594-597, 1904. 



IRON ORGANISMS 



Medium 61 



Solution for Thread Bacteria 



Potassium acetate (KC 2 H 3 O 2 ) 0.5 gm. 



Ferrous carbonate (FeCOs) . : 0.5 gm. 



Tap-water 1000.0 c.c. 



Medium 62 

 Solution for Thread Bacteria 



Ferrous ammonium citrate (about 16 per cent. Fe) 0.5 gm. 

 Tap-water 1000.0 c.c. 



Medium 63 



Solution for Isolating Chlamydothrix 



(a) Agar 10.0 gm. 



Manganese peptone (4 per cent. Mn 2 O 3 ) 0.5 gm. 



Tap-water 1000.0 c.c. 



(b) Gelatin 100.0 gm. 



Manganese peptone (4 per cent. Mn 2 O 3 ) 0.25 gm. 



Peat extract 1000.0 c.c. 



Make the reaction slightly alkaline with normal potassium 

 hydroxid. 



