122 SOIL BACTERIOLOGY 



Medium 73 



Clover Agar 



Clover (green) 500.0 gm. 



Agar 25.0 gm. 



Saccharose (CwHaOu) 2.0 gm. 



Potassium nitrate (KNO 3 ) 0.5 gm. 



Tap-water 1000.0 c.c. 



Extract the clover tissue by heating for one hour in the 

 steamer; filter and add the other ingredients. Add i to 2 



drops of N/io hydrochloric acid to each tube of the medium 

 just before pouring plates. 



ACTINOMYCETES 



Medium 74 

 Solution for Actinomycetes 



Dibasic potassium phosphate (K 2 HPO4) 0.5 gm. 



Potassium nitrate (KNO 3 ) 3.0 gm. 



Calcium malate (CaC 4 H 4 O 5 )2 + 6H 2 O) 10.0 gm. 



Tap-water 1000.0 c.c. 



Krainsky, A., Centbl. Bakt. (etc.), Abt. 2, Bd. 41, pp. 649-688, 1914. 



Medium 75 



Agar for Actinomycetes 



Agar 15.0 gm. 



Glycerin 10.0 gm. 



Sodium asparaginate (NaC 4 H 6 NO 4 + H 2 O) i.o gm. 



Glucose (C 6 Hi 2 O 6 ) i.o gm. 



Ammonium hydrogen phosphate (NH4H 2 PO 4 ). ... 1.5 gm. 



Magnesium sulphate (MgSO 4 .+ 7H 2 O) 0.2 gm. 



Calcium chlorid (CaCl 2 ) fused o.i gm. 



Potassium chlorid (KC1) o.i gm. 



Ferric chlorid (FeCl 3 + 6H 2 O) trace 



Distilled water , 1000.0 c.c. 



Conn, H. J., Jour. Bact., vol. i, p. 198, 1916. 



