126 SOIL BACTERIOLOGY 



(6) Calcium nitrate (Ca(NO 3 )2) '42.7 gm. 



Distilled water 1000.0 c.c. 



(c) Magnesium sulphate (MgSO 4 + 7H 2 O) go. 2 gm. 



Distilled water 1000.0 c.c. 



(d) Ferric phosphate (FePO 4 + 4H 2 O) . . . 2.2 gm. 



Distilled water. 1000.0 c.c. 



To prepare the complete nutrient solution, take 2o-c.c. 

 portions of (a), (b), and (c) and dilute to i liter; now add 

 0.5 c.c. of solution (d). 



Shive, J. W., Physiological Researches, Vol. I, No. 7, Johns Hopkins 

 University, pp. 327-397, 1915. 



Medium 84 

 Solution Similar to Sea-water 



Sodium chlorid (Nad) . 26.0 gm. 



Magnesium chlorid (MgCl 2 + 6H 2 O) 3.7 gm. 



Potassium chlorid (KC1) i.o gm. 



Magnesium sulphate (MgSC>4 + 7H 2 O) 1.7 gm. 



Calcium sulphate (CaSO* + 2H 2 O) i.o gm. 



Distilled water 1000.0 c.c. 



This solution is recommended for the cultivation of organ- 

 isms accustomed to sea-water. 



Medium 85 



Agar for Preserving Plate Cultures 



Washed agar 20 gm. 



Glycerin (C 3 H 6 (OH) 3 ) 500 c.c. 



Distilled water 500 c.c. 



Dissolve the agar in the water by heating in a steamer, add 

 the glycerin, and filter through glass wool. 



