SECTION VIII 



PREPARATION OF STAINS 



PREPARE saturated alcoholic solutions of methylene-blue, 

 gentian- violet, and fuchsin. 



1. Place the dye in a large test-tube (about one-fourth 

 full) and fill with 95 per cent, alcohol. 



2. Let stand twenty-four hours and shake from time to 

 time. 



(1) Loffler's Methylene-blue: 



Saturated alcoholic solution of methylene-blue 30 c.c. 



Potassium hydroxid in distilled water .(i : 10,000) ... 70 c.c. 



(2) Gentian-violet (Aqueous): 



Saturated alcoholic solution of gentian- violet 5 c.c. 



Distilled water 95 c.c. 



(3) Carbol-fuchsin: 



Saturated alcoholic solution of fuchsin 10 c.c. 



Aqueous solution of carbolic acid (5 per cent.) 90 c.c. 



(4) Gram's lodin Solution: 



Metallic iodin i gm. 



Potassium iodid 2 gm. 



Water 300 c.c. 



Dissolve in a few cubic centimeters of water. When in 

 solution bring volume to 300 c.c. 



137 



