BACTEKIA, DESTRUCTIVE INFLUENCES 



41 



properly kept. It is extensively used to wash out 

 abscess-cavities and purulent sinuses, and also as a 

 gargle and mouth-wash. 



Demonstration. The teacher should inoculate a loopful from a 

 twenty-four-hour broth culture of typhoid (or colon) bacilli into the 

 following tubes: and make subcultures from each of these tubes at 



10 c.c. 



Carbolic 



Fig. 18. 



the end of one minute or five minutes by inoculating a loopful from 

 these tubes into plain sterile broth. 



Instead of making broth subcultures, agar poured plate cultures 

 can be made. 



Dakin's Solution. 1 Experience having shown that there 

 were certain drawbacks to Dakin's original formula, the 

 following method of preparing the Dakin hypochlorite 

 solution used by Carrel was devised by Daufresne. 



To prepare 10 litres of solution weigh out exactly: 

 Chloride of lime (having 2 per cent, active chlorine) .... 184 grams. 

 Carbonate of soda, anhydrous (or carbonate of soda, 



crystals, 262 grams) 92 grams. 



Bicarbonate of soda 76 grams. 



Into a 12-litre flask place the chloride of lime and 5 



1 This is taken, by permission, from "The Treatment of Infected 

 Wounds," by A. Carrel and G. Dehelly. New York, Paul B Hoeber, 

 1917. 



