INTRODUCTION. 



ounces, serves very well for injection. A cannula of 

 correct size may be had by asking the druggist for the 

 filling cannula of the Parke Davis serum 

 syringe. The end to be inserted into 

 the blood-vessel should be ground off 

 obliquely and smoothly on a whetstone. 

 The connection between the cannula and 

 nozzle is formed by stiff rubber tubing 

 which should be securely tied to the can- 

 nula (Fig. 2). 



The injecting mass is prepared by 

 thoroughly mixing 100 c.c. of water, 20 

 c.c. of glycerin, 20 c.c. of concentrated 

 formalin, and 85 gm. of common laun- 

 dry starch. One-half should be colored 

 red by adding to it one or two grams of 

 powdered carmin made into a paste with 

 a few drops of ammonia, and the other 

 colored blue by dissolving in it a gram 

 or two of soluble Berlin blue. These 

 liquid masses, after being passed through 

 a fine wire strainer or a coarse piece of 

 cheese-cloth stretched across a funnel, 

 may be preserved for any length of time 

 in covered jars. 



The animal may be anesthetized by 

 placing it in a tight box with a cloth or 

 absorbent cotton wet with 30 c.c. of 

 ether or chloroform. If only the ar- 

 teries are to be injected, the cat may 

 remain in the box until dead; but if the 

 veins are also to be injected, it is preferable to use ether for 

 the anesthetic and then remove the animal to the tray as 

 soon as it is unconscious, and reflect a portion of skin, ex- 



FIG. 2. INJECTING 

 SYRINGE. X &. 



p, Plunger handle ; 

 b, barrel; pi, 

 nozzle ; c, can- 

 nula ; r, rubber 

 tube ; dt, point 

 of detachment ; 

 s, string. 



