INTRODUCTION. 



sel as in the case of the jugular. Lift up the artery with 

 the index finger (Fig. 4), and make an oblique incision 

 with the scissors. The cannula with the connecting tube 

 attached should then be inserted caudad in the oblique cut 

 of the artery (Fig. 5), and the string 

 s drawn tight so as to hold the cannula 

 in place. The string st should then 

 be tightened to prevent the injection 

 mass from running out where the can- 

 nula is inserted. After stirring the 

 red mass, filling the syringe, and slip- 

 ping the nozzle into the connecting 

 tube the operator should press slowly 

 but continuously on the plunger until 

 that portion of the carotid craniad of 

 the string st is well distended. In a 

 large cat this will not occur until the 

 syringe is nearly or quite empty. 

 When the vessels are full, the can- 

 nula is withdrawn while the string ^ is 

 tightened. The syringe is washed out, after which the blue 

 mass is well stirred and injected caudad into the external 

 jugular in the same manner as described for the arteries. 



By making a slit about three inches long in the abdominal 

 wall, a fold of the intestine may be pulled out so that its 

 lumen can be filled with about 200 c.c. of 15% formalin. 

 The same amount should be injected into the lungs by 

 inserting the cannula caudad into the ventral wall of the 

 trachea. If the cat is not to be used at once, it may be 

 preserved indefinitely in a jar of five per cent, formaline. 



The portal system is not injected through the jugular 

 vein. The portal trunk just caudad of where it enters the 

 liver may be found by cutting a piece from the abdominal 

 wall on the right ventral aspect just caudad of the last rib. 



FIG. 5. METHOD OF 

 INSERTING THE CAN- 

 NULA INTO A VES- 

 SEL. 



