ELEMENTS OF MAMMALIAN ANATOMY. 



After two loose knots are tied around this trunk as directed 

 for the jugular, the cut is made and the cannula inserted 

 caudad. About 15 c.c. of the blue mass may be injected 

 into the portal system of a large specimen. This should 

 not be injected until the other systems have been filled. 



The lymphatic system must likewise be injected for 

 demonstration. While the cat is being anesthetized, 5 gm. 

 of soluble Berlin blue are dissolved in 100 gm. of water, 

 and the solution warmed to about the 

 temperature of the body. As soon as 

 the cat is unconscious the syringe should 

 be warmed by filling it with hot water, 

 and the point of the cannula pushed ob- 

 liquely proximad under the thick skin on 

 the palm (Fig. 6) of the paw. The 

 syringe after being half filled with Berlin 

 FIG. 6. PALMAR klue solution is attached to the cannula 



ASPECT OF CAT'S and the plunger pushed in very slowly 

 PAW WITH CAN- - . . . . / 



NULA INSERTED. t . so tnat one- fourth of the amount in the 



syringe is forced out in ten minutes. 

 While the injection is being made the limb should be gently 

 massaged by pinching and rubbing from the foot toward 

 the body. This facilitates the flow of the liquid in the 

 lymph-vessels. In this manner the lymphatics of each limb 

 are filled. The author has been able to fill the left thoracic 

 duct by continuing the injection for about twenty minutes 

 in the palm of the left paw. The lymphatics of the head 

 and neck may be injected in a similar manner by inserting 

 the cannula beneath the skin on the tip of the ear, the tip 

 of the tongue, the tip of the nose, and the lips. 



In order to inject the lacteals the abdominal cavity must 

 be opened, and the cannula inserted very obliquely into the 

 wall of the small intestine so that its point is between the 

 mucous and muscular coats. The half -filled syringe being 



