VI 



CONTENTS 



PAGE 



Exercise 19. Method of Measuring Microorganisms 70 



Exercise 20. Determination of the Rate of Movement of Motile 



Organisms 74 



Exercise 21. Preparation of a Hanging Drop 74 



Exercise 22. Preparation of the Adhesion Culture 78 



Exercise 23. Preparation of the Moist-chamber Culture 80 



Exercise 24. Preparation of the Agar Hanging-block Culture .... 81 

 Exercise 25. Lindner's Concave-slide Method of Demonstrating 



Fermentation 83 



Exercise 26. Lindner's Droplet Culture 84 



Exercise 27. Chinese Ink Preparation. . 85 



Exercise 28. The Staining of Microorganisms 87 



Exercise 29. Anjeszky's Method of Staining Spores 90 



Exercise 30. Method of Staining Tubercle and Other Acid-fast 



Bacteria 92 



Exercise 31. Method for Staining Flagella 93 



Exercise 32. Gram's Method of Staining 95 



Exercise 33. Method for Staining Capsules 97 



Exercise 34. Method of Making Impression Preparations 98 



Exercise 36. Method of Staining the Nuclei .of Yeast Cells 99 



General Characteristics of Mold Growth 100 



Exercise 36. Microscopical Examination of Molds 105 



Exercise 37. The Study of Molds 107 



Exercise 38. To Determine the Acidity Changes Produced by 



, Molds in Cider 112 



Exercise 39. To Demonstrate the Pathogenic Nature of Molds. . 113 



Yeasts - 114 



Exercise 40. To Isolate a Pure Culture of Saccharomyces Cere- 

 visiaB and to Study the Flora of a Compressed 



Yeast Cake 114 



Exercise 41. Apparatus and Methods for the Study of Gaseous 



Fermentation 117 



Exercise 42. The Study of Yeasts 119 



Exercise 43. The Study of Bacteria 125 



Exercise 44. Ehrlich's Method of Testing Indol Production 132 



Exercise 45. Tests for the Reduction of Nitrates 133 



Descriptive Chart Society of American Bacteriologists 134 



Exercise 46. To Demonstrate the Efficiency of Intermittent 



Heating as a Method of Sterilizing Media 139 



Exercise 47. To Compare Morphologically Protozoa with Bac- 

 teria 140 



Exercise 48. To Study the Natural Decomposition of Milk 141 



