CONTENTS 



PART I 



GENERAL MORPHOLOGICAL AND CULTURAL 

 METHODS 



PAGE 



Exercise 1. Cleaning Glassware 1 



Sterilization 5 



Exercise 2. Preparation of Glassware for Sterilization 15 



Nutrient Media 18 



Exercise 3. Titration of Media 20 



Milk 23 



Exercise 4. Preparation of Litmus Milk 25 



Exercise 5. Preparation of Glycerin Potato 26 



Exercise 6. Preparation of Meat Infusion 28 



Exercise 7. Preparation of Nutrient Broth 29 



Gelatin 31 



Exercise 8. Preparation of Nutrient Gelatin 36 



Agar : . . . 37 



Exercise 9. Preparation of Nutrient Agar 41 



Exercise 10. Preparation of Dunham s Peptone Solution 43 



Exercise 11. Nitrate Peptone Solution 44 



Cultures 44 



Exercise 12. Preparation of Plate Cultures, Loop or Straight 



Needle Dilution Method 49 



Exercise 13. Preparation of Plate Cultures, Quantitative Dilu- 

 tion Method 52 



Exercise 14. Methods of Counting Colonies in Petri Dish Cul- 

 tures 56 



Exercise 16. Isolation of Microorganisms from Plate Cultures 



and Method of Making Agar Streak Culture. ... 58 

 Exercise 16. Method of Making Transfers of Pure Cultures into 



a Liquid Medium 60 



Exercise 17. Method of Making Stab Cultures 61 



Exercise 18. Preparation of a Giant Colony 61 



The Microscope , . . . 63 



