IV PREFATORY NOTE 



intimation that the special iields have not been admirably 

 dealt with by others. 



The subject matter given under Part I of this manual is 

 primarily for the purpose of giving a working knowledge of 

 laboratory methods used in the study of microorganisms. 

 Molds, yeasts and bacteria are taken up in the order of their 

 comparative sizes and studied as to their identification by 

 morphological and cultural methods. It is presupposed that 

 the student has a knowledge of these microorganisms 

 acquired from preceding lectures in microbiology. 



Part II consists of exercises demonstrating the various 

 physiological activities of microorganisms. 



Part III deals with applied microbiology. After the 

 student has familiarized himself with the ordinary tools and 

 technic, etc., as dealt with in Parts I and II, it is not neces- 

 sary that he be burdened with minute, detailed instructions. 

 We have had this in mind in preparing Part III. 



No attempt has been made to compile an exhaustive 

 list of exercises; the aim has been only to cover a wide 

 range of activities under each different subject. In many 

 cases, exercises have been taken directly, with few or no 

 modifications, from laboratory manuals already in print. 

 Credit has not been given directly; the list of references, 

 however, includes all books from which material has been 

 taken. 



The purpose of this laboratory manual is to make the 

 student more independent. Practically all directions for 

 work to be done are contained in it; for this reason the work 

 as assigned from day to day should be read -over carefully 

 before beginning an exercise and then followed step by step. 

 Any desirable changes in directions may be indicated by 

 the instructor. 



WARD GILTNER, 



Head of Department, 

 EAST LANSING, MICH. 

 Sept. 1, 1915. 



