PREFATORY NOTE 



LABORATORY instruction in bacteriology at the Michigan 

 Agricultural College developed under the direction of Dr. 

 C. E. Marshall. This laboratory guide represents the accu- 

 mulated efforts of instructors working for a period in excess 

 of a decade. To Assistant Professor L. Zae Northrup is 

 due the credit for collecting and arranging the material pre- 

 sented as well as for preparing de novo many of the experi- 

 ments and much of the supplementary matter. She has 

 been assisted by Mr. W. L. Kulp. Dr. E. T. Hallman and 

 Dr. L. R. Himmelberger have taken the responsibility for 

 arranging the exercises relating to immunity, serum therapy 

 and pathologic bacteriology. Great praise is due Dr. F. H. 

 H. Van Suchtelen for introducing many new features into 

 the laboratory work during the academic year 1912-13, 

 and also Dr. Otto Rahn for his several years of admirable 

 effort immediately preceding. Others whose influence has 

 been felt in creating this guide and to whom credit is due 

 are Professors W. G. Sackett, S. F. Edwards, L. D. Bush- 

 nell, C. W. Brown and W. H. Wright. 



While some claim to originality may be made for this 

 laboratory guide, it is to be expected that much of the 

 material herein has been presented in various other manuals 

 and perhaps in better form in many instances. The greatest 

 effort has been made to make this a laboratory guide to 

 General Microbiology, leaving the particular fields of dairy, 

 soil, water, medical and other phases of bacteriology to 

 special guides already in print or at present projected. The 

 presentation of this manual to the public is in no way an 



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