78 



GENERAL MICROBIOLOGY 



EXERCISE 22. PREPARATION OF THE ADHESION 

 CULTURE 



The purpose of the exercise is to show the germination 

 of mold spores or the budding of yeast cells, i.e., colony 

 formation. 



Apparatus. Clean cover-glasses; clean concave slides; 

 melted paraffin; small glass rod or camel's-hair brush; 



FIG. 



28. Lindner's Adhesion Culture. (Adapted from Lafar's 

 Technische Mykologie.) 



sterile cider in tubes. (Wort, milk and other media may be 

 used as conditions demand.) 



Cultures. Pure culture of mold, or yeast. If mold 

 spores are to be germinated, an old culture having spores is 

 necessary. 



Method. 1. Inoculate a tube of sterile cider from the 

 pure culture of the organisms to be studied. (Use spores 

 in the case of mold and some of the cells for yeasts.) Dis- 

 tribute well with the platinum needle. 



2. Transfer one loopful to a flamed cover-glass and 

 spread in a thin film over the entire surface of the cover- 

 glass, using the straight needle. If any of the cider adheres 

 in droplets, shake them off. 



