94 GENERAL MICROBIOLOGY 



distilled water; mordant for flagella staining; anilin-water 

 fuchsin or gentian violet. 



Culture. Agar slant culture, twelve to eighteen hours old. 



Note. The best results are obtained if successive generations of 

 this organism have been transplanted every eighteen to twenty-four 

 hours for several generations. 



Method. 1. Place three drops of distilled water on a 

 clean glass slide. 



2. Transfer a small amount of bacterial material from 



- FIG. 34. Flagella Stain. B. typhosus, X1500. 



the moist portion of the agar slant culture to the first drop 

 by means of a small platinum loop, using only enough of 

 the material to make this drop very slightly cloudy. 



3. Flame the needle and transfer a small portion from 

 the first drop to the second. 



4. Proceed in like manner in preparing the third dilu- 

 tion. 



5. Place a number of absolutely clean cover-glasses in 

 cover-glass forceps. 



6. By means of a platinum needle bent at right angles 

 near the end, make smears on the cover-glasses from the 



