THE STUDY OP BACTERIA 129 



motility, spores. Use ocular No. 1 and objective No. 7. 

 The greatest motility will be observed in organisms growing 

 in the condensation water at the base of the slant. 



3. Draw the total organism and record the presence or 

 absence of motility. Describe all cultures at the time the 

 drawings are made of each, following the terminology of 

 the " Descriptive Chart of the American Society of Bac- 

 teriologists," p. 134. 



4. Use drawing pencil for making drawings and ink for 

 recording descriptions. 



Any descriptive terms may be added which will aid in 

 identifying organisms, but descriptive chart terms must 

 be followed as closely as possible, otherwise drawings will 

 not be accepted. 



Always state the age of the culture, the temperature at 

 which the organism is grown, the medium upon which it is 

 cultivated and the litre of the medium. 



Use one chart for each organism. 



5. When the agar slant culture of each organism shows 

 good growth, make inoculations from this culture into the 

 following media : 



Agar plate (see below for method). 



Gelatin plate (see step 6, below). 



Nutrient broth. 



Nutrient gelatin (stab culture). 



Litmus milk. 



Glycerin potato. 



Dunham's solution. 



Nitrate peptone solution. 



Plain broth fermentation tube (control). 



Dextrose broth fermentation tube. 



Lactose broth fermentation tube. 



Saccharose broth fermentation tube. 



6. In preparing agar plate from bacterial cultures, proceed 

 as follows: Inoculate a tube of nutrient broth lightly, using 



