158 



GENERAL MICROBIOLOGY 



II. EXHAUSTION OF AIR 



A. By Boiling. It is well to expel all the air from a 

 medium to be used for isolating or growing anaerobes by 

 boiling twenty to thirty minutes, and cooling rapidly just 

 previous to inoculating, and placing under anaerobic con- 

 ditions. 



B. Cultivation in Vacuo. This requires special apparatus 

 for obtaining a vacuum and for cultivation in some cases. 



FIG. 39a. Novy Jar for 

 Tube Cultures. 



FIG. 396. Novy Jar for 

 Plate Cultures. 



Apparatus. Special tubes: 



1. Vacuum tubes (Fig. 129, p. 238, Eyre's Bacteriological 

 Technic) . 



2. Pasteur, Joubert and Chamberland's tube (Fig. 80, 

 p. 93, Besson's Practical Bacteriology, Microbiology and 

 Serum Therapy). 



3. Pasteur's tube (Fig. 81, Besson, ibid.). 



4. Lacomme's tube (Fig. 82, Besson, ibid.). 



5. Roux's tube for stroke cultures (Fig. 91, p. 101, 

 Besson, ibid.). 



6. Roux's tube for potato cultures (Fig. 92, p. 101, 

 Besson, ibid.). 



7. Esmarch's tube (Fig. 95, p. 103, Besson, ibid.). 



8. Vignal's tube (Fig. 96, p. 103, Besson, ibid.). 



