ANAEROBIC CULTURE METHODS 



159 



Special flasks: 



1. Pasteur's flask (Fig. 79, p. 92, Besson, ibid.). 



2. Flasks with long necks (Fig. 83, p. 94, Besson, ibid.). 



3. Bottle (Fig. 84, p. 94, Besson, ibid.). 



4. Kitasato's dish (Fig. 93, p. 10, Besson, ibid.). 



5. Bombicci's dish (Fig. 94, p. 102, Besson, ibid.). 



6. Ruffer's or Woodhead's flask (Fig. 33, p. 41, Eyre, 

 ibid.). 



Special jars in which test tube or plate cultures may be 

 placed and a vacuum produced. 



FIG. 40. Bulloch's Anaerobic Jar. 



1. Novy's jar for plates (Fig. 135, p. 245, Eyre, ibid.). 



2. Novy's jar for tubes (Fig. 136, p. 245, Eyre, ibid.). 



3. Bullock's anaerobic apparatus (Fig. 137, p. 247, Eyre, 

 ibid.). 



4. Tretrop's apparatus (Fig. 97, p. 105, Besson, ibid.). 



5. Botkin's apparatus (Fig. 134, p. 244, Eyre, ibid.). 

 Apparatus for obtaining a vacuum: 



1. Electric pump adaptable to vacuum or pressure. 



2. Water vacuum pump. 



3. Mercury vacuum pump. 



Method. 1. The tube and flask cultivations are prepared 

 by, (a) placing the desired medium in the vessel ; (6) inoculat- 

 ing from the desired source; (c) attaching to the vacuum 



