ANAEROBIC CULTURE METHODS 



161 



3. Buchner's tube (Fig. 130, p. 239, Eyre, ibid.). 



4. Turro's tube (Fig. 86, p. 95, Eyre, ibid.). 



By the use of these tubes no sealed jar is necessary. 



FIG. 41. Giltner's Tube. (Orig.) FIG. 42. Buchner's Tube. 



1. The simple test-tube method is advantageous in that 

 it requires no special apparatus. It has disadvantages, how- 

 ever, which will be mentioned later. 



Apparatus. Test tube of sterile medium; rubber stopper 

 to fit tube; pyrogallic acid and sodium hydroxide; paraffin. 



