216 GENERAL MICROBIOLOGY 



4. Determine the weight at once and then every day 

 until the weight becomes constant. 



5. Inoculate flask B with Bad. aceti and flask C with both 

 Bad. aceti and Sacch. ellipsoideus. 



6. Titrate B and C every two days. Titrate flask A 

 only at the end of the experiment. 



7. Determine the amount of alcohol formed in flask A 



FIG. 46. Antibiosis. This peculiarity of growth is the result of the 

 inhibitive action of metabolic products diffused through the 

 medium. (Orig. Northrup.) 



by distilling the contents of the flask and determining the 

 specific gravity of the distillate. 



How much CO2 was given off? Calculating from this 

 amount, how many grams of sugar (CeH^Oe) were present 

 in the flask? What percent sugar was this solution? 



What was the theoretical amount of alcohol present? 



8. Plot curves showing the acid formation in each 

 case. Explain the direction which these curves take. 



