ANIMAL INOCULATION IN BACTERIOLOGY 295 



ANIMAL DISEASES AND IMMUNITY 



EXERCISE 1. ANIMAL INOCULATION IN BACTERIOL- 

 OGY FOR DETERMINATION OF THE IDENTITY 

 OF A MICROORGANISM, ITS PATHOGENICITY OR 

 VIRULENCE, OR FOR PRODUCTION OF IMMUNITY 



Apparatus. Experimental animals: rabbits; guinea 

 pigs; white rats; white mice, etc.; scalpels; scissors; 

 forceps; razor; syringe; trephine; sterile dishes; anes- 

 thetic; disinfectant; cotton. 



Culture. Pure culture or infected material. 



I. INTRODUCTION 



1. Avoid the use of animals where the employment of 

 other means answers the purpose equally well. 



2. Unless other factors prevent, always use the most 

 susceptible and least expensive animals. 



II. PREPARATION OF ANIMAL * 



Method. 1. (a) Examine carefully each animal before 

 subjecting it to experimentation. 



(6) Use no animal already showing symptoms of illness 

 or general lack of vigor. 



(c) Record the weight and temperature of each animal. 



2. (a) Administer an anesthetic (general or local as 

 indicated) whenever the operation is very painful or tedious 

 or where perfect immobility of the parts is required. 



Note. For local anesthesia a 2% solution of cocaine hydrochloride 

 may be made by dissolving 0.1 gm. of cocaine hydrochloride in 5 c.c. 

 of sterile water. Instill a few drops into the conjunctiva! sac or inject 

 1 to 5 c.c. into the subcutaneous tissues near the seat of operation. 

 For general anesthesia 10 to 30 c.c. of a 5% solution of chloral hydrate 

 may be injected per rectum, or ether or chloroform may be inhaled. 



*The instructor must arrange for experiments that must be started 

 early in order to be completed before the term closes. 



