296 



GENERAL MICROBIOLOGY 



Ether is probably safer in the hands of a novice. It may be adminis- 

 tered by saturating cotton placed in a paper cone which is kept over 

 the animal's nose. Care should be exercised to replenish the supply 

 of the anesthetic on the cotton as fast as it volatilizes and not to force 

 the anesthetizing too fast. Injury to the integument about the 

 nose may be avoided by rubbing on vaseline before beginning the 

 operation. The tissues should not be cut until anesthesia is complete. 



(6) Choose a site for operation where the results will not 

 interfere with the animal's locomotion or normal functions, 

 (c) Use sharp, sterile instruments. 



FIG. 63. Tray for Sterilizing Surgical Instruments. 



Note. Methods for holding different animals for different forms 

 of operations vary. An assistant is usually required to hold the animal, 

 where an anesthetic is not administered, and where an anesthetic is 

 used it is usually better to have an assistant administer it, although 

 this is not necessary. (For various devices for holding experimental 

 animals see text-book: Eyre, Bacteriological Technic, 2d Ed. (1913), 

 pp. 349-352.) 



3. Remove the hair with scissors or clippers from the 

 field of operation and shave the surface. Wash the skin 

 and disinfect it with 2% liquor cresolis compositus (U. S. P.). 

 Wash off the disinfectant with alcohol and allow the 



