ISOLATION OF PATHOGENIC BACTERIA 301 



Note. Fasting the animal before introducing unpalatable material 

 into the food may be helpful in increasing the amount eaten. The 

 chemical reaction of the stomach contents as governed by physio- 

 logical activity will influence results. 



IV. CARE OF INOCULATED ANIMALS 



1. Watch each animal closely and take temperatures as 

 the case demands. 



2. Treat each animal as a case of infectious disease in 

 quarantine. 



Note. Whatever clinical, diagnostic or sanitary measures neces- 

 sary in that given disease may be employed as seen fit. 



3. When the animal is removed from the cage for the 

 last time, carefully destroy all refuse in the cage and dis- 

 infect thoroughly. 



4. Give all results, observations and conclusions in 

 detail. 



REFERENCES 



STITT: Practical Bacteriology, Blood Work and Parasitology, 3d 



Ed., 1914, pp. 48, 143, 152. 

 MOORE and FITCH: Bacteriology and Diagnosis, pp. 114,' 118-120, 



124, 125, 130, 133. 



EYRE: Bacteriological Technic. pp. 332-369. 

 KOLMER: Infection, Immunity and Specific Therapy (1915), pp. 53-64. 



EXERCISE 2. THE ISOLATION OF PATHOGENIC BAC- 

 TERIA FROM FLUIDS AND TISSUES OF DEAD 

 ANIMALS 



Apparatus. Disinfectant; scalpel; scissors; forceps; 

 bone forceps; ten sterile pipettes; 10 c.c. sterile pipettes; 

 250 c.c. flask containing glass beads, sterile; sterile Esmarch 

 dishes; spatula; platinum loop; special media. 



Method. 1. Disinfect the skin. 



2. Remove the spleen, kidney, lymph glands, and any 

 other diseased tissue, to sterile Esmarch dishes, using sterile 

 instruments. 



