302 GENERAL MICROBIOLOGY 



3. Collect samples of pericardial and pleuritic fluids* 

 blood, urine and bile with sterile pipettes and place 

 these in small sterile flasks. Collect at least 25 c.c. of 

 blood in a sterile flask containing glass beads for defibrinat- 

 ing. 



4. Remove the organs collected to the laboratory and 

 make cultures as follows: 



5. Sear the surface of the organ with a spatula heated to 

 a white heat. 



6. Tear the seared surface with forceps, sterilized in 

 flame. 



7. With a sterile platinum loop, make transfers to agar 

 slants, shake cultures, and plates for isolation into pure 

 cultures. 



8. Repeat 7, using any body fluids collected. 



Note. The different diseases require special procedures and 

 media for successful results. Attention will be called to these varia- 

 tions at the proper places. 



REFERENCES 



MOORE and FITCH: Bacteriology and Diagnosis, pp. 95-96. 

 MOORE: Principles of Microbiology, pp. 156-162, 237-258. 

 EYRE: Bacteriological Technic, pp. 248-258. 



EXERCISE 3. A STUDY OF BACT. ANTHRACIS 



Note. Bact. anthracis is the cause of anthrax, a disease very fatal 

 to man and certain domestic animals. Great care should be taken 

 while working with it. 



Apparatus. Six tubes of agar; three tubes of potato; 

 three tubes of milk; tube of gelatin; slides and stains; 

 autopsy instruments. 



Culture. Bact. anthracis. 



Method. 1. Inoculate three tubes each of agar, potato^ 

 and milk and one tube of gelatin with Bact. anthracis. 



