312 



GENERAL MICROBIOLOGY 



4. Shake all tubes well and incubate at 37 C. for twenty- 

 four hours and record the results. 



Microscopic Test. 1. If this test is carried out during 

 the same period with the macroscopic test, a small loopful 

 of the dilution from any tube may be transferred to a clean 

 cover-glass placed on a hanging drop slide and the edges 

 sealed with vaselin or oil. It may then be examined with 

 a microscope. 



2. If done independently of the macroscopic test, prepare 

 the suspension of organisms in one test tube and the dilu- 

 tions of serum in others. 



Mix a loopful of the diluted serum with a loopful of the 

 antigen on a clean cover-glass, mount on a concave slide 

 and observe with a microscope for a period of thirty minutes 

 to one hour. 



3. Give results and any conclusions in detail. 



REFERENCES 



KOLMER: Infection, Immunity and Specific Therapy (1915), pp. 68, 



69, 79, 152, 266-291. 



MARSHALL: Microbiology (1911), pp. 488, 567-570. 

 MCFARLAND: Pathogenic Bacteria and Protozoa, 7th Ed. (1912), 



pp. 149-152. 

 GELTNER: Studies of Agglutination Reactions in Hog Cholera during 



the Process of Serum Production (Preliminary) Tech. Bui. 3 



(1909), Mich. Agr. Expt. Sta. 



GILTNER: Same title Tech. Bui. 8 (1911), Mich. Agr. Expt. Sta. 

 ZINSSER: Infection and Resistance (1914), pp. 218-247. 



