316 GENERAL MICROBIOLOGY 



GILTNER: What is the Antigen Responsible for the Production of 

 Antibodies in Hog Cholera Serum? Tech. Bui. 13, Mich. Agr. 

 Expt. Sta. 



KOLMER: Infection, Immunity and Specific Therapy (1915), pp. 77, 78. 



EXERCISE 10. THE PREPARATION OF BACTERINS OR 

 BACTERIAL VACCINES 



Apparatus. Scalpel; scissors; forceps; sterile tubes 

 and Esmarch dishes; sterile swabs; sterile physiological 

 salt solution; 50% alcohol; disinfectant; four agar slants; 

 six agar tubes for plating; six sterile Petri dishes. 



Culture. Infected material or specific cultures to be 

 furnished by the instructor. 



A. AUTOGENOUS BACTERINS 



Method. In the preparation of an autogenous bac- 

 terin, it is first necessary to isolate the microorganism 

 causing the disease. This is accomplished as follows: 



1. If there are any unopened abscesses, open one with 

 a sterile scalpel after first disinfecting the field with 2% 

 compound solution of cresol an4 washing with 50% alcohol. 

 Collect some of the pus on a sterile swab and suspend 

 in sterile 'physiological salt solution. 



2. If the abscess is already opened, using a sterile 

 curette, obtain some of the diseased tissue at the bottom 

 of the abscess and macerate this in sterile physiological 

 salt solution. 



3. Pour agar plates from this salt solution suspension, 

 using at least six, plated in series of two. 



4. Incubate the plates and after twenty-four hours 

 make observations on the number and type of colonies. 

 After forty-eight hours make transfers to agar slants of the 

 most numerous type of colony. Colonies should be studied 

 under low power of the microscope. 



5. Grow three or four cultures of the organism on slanted 



