328 



GENERAL MICROBIOLOGY 



HEMOLYTIC SYSTEM 



Incubate half an hour in a water bath at 37 C. and then keep in the ice box for 

 twelve hours and read. 



A. 0.9% salt solution. 



B. Inactivated serum known to contain antibodies. 



C. Suspension of a culture of suspected bacteria, carbolized. 



D. Titrated guinea pig serum diluted so that 0.1 c.c. contains 1.5 times the 

 titre. 



E. Immune rabbit serum of known titre diluted so that 0.1 c.c. contains three 

 times the titre. 



F. 1% suspension of washed sheep blood cells in salt solution. 



G. * signifies a variable reaction according to the activity of t 

 + signifies a complete hemolysis. 



signifies no hemolysis. 



The smallest quantity of antigen (in combination with antibody) that com- 

 pletely fixes the complement is known as the litre. 



ity of the antigen. 



II. COMPLEMENT FIXATION TEST. (Test proper) 



1. Suspected Serum. 'This is drawn from' the animal 

 that is suspected of being infected with the infectious 

 disease in question. 



The blood is drawn from the jugular vein. It is allowed 

 to clot and the serum collected. It must be inactivated 

 before testing unless it is to be held for a week or more 

 before applying the test, in which case inactivation is not 

 necessary, but 1% phenol should be added as a preserva- 

 tive. 



Note. The test proper and controls must be run at the same time. 

 If several tests are run at the same time one set of controls is sufficient. 



2. Test of Suspect Serum. 



Incubate one-half hour at 37 C, in water bath. 



