THE COMPLEMENT FIXATION TEST 



329 



3. Then add the hemolytic system. 



Incubate one-half hour at 37 C. in water bath and then place in ice box for 

 twelve hours and read. 



A. Salt solution, 0.9%., 



B. Suspect ser\im inactivated for one-half hour. 



C. Antigen, two times titre. 



D. Complement, 1.5 times titre. 



E. 1% washed sheep blood corpuscles in salt solution. 



P. Immune rabbit serum (hemolysin) diluted so that 0.1 c.c. contains three 

 times the titre. 



Control tubes 2 and 3 should show complete hemolysis. 

 Control tubes 1 and 4 should show complete absence of hemo- 

 lysis. Control tube 4 is control on the inactivation of the 

 suspect's serum and should show absence of hemolysis. 



Hemolysis in the other tubes indicates the absence of 

 antibodies in sufficient quantity in the amount of serum 

 used to fix complement. 



4. Give all results in detail and draw conclusions. 



REFERENCES 



MOHLER, J. R. and EICHHORN, A.: The diagnosis of glanders by 

 complement fixation test. Bui. 136, B. A. I., U. S. Dept. Agr. 

 (1911). 



SURFACE, F. M.: The diagnosis of infectious abortion in cattle. 

 Bui. 166, Kentucky Agr. Expt. Sta. (1912). 



HADLEY, F. B. and BEACH, B. A.: The diagnosis of contagious abor- 

 tion in cattle by means of the complement fixation test. Res. 

 Bui. 24. Wis. Agr. Expt. Sta. (1912). 



MOHLER, JOHN R., EICHHORN, A. and BUCK, J. M.: The diagnosis 

 of dourine by complement fixation. Jour. Agr. Res. Bui., Vol. I 

 (1913), pp. 99-109. 



KOLMER: Infection, Immunity and Specific Therapy (1915), pp. 146, 

 164, 185, 316-501, 847-863. 



ZINSSER: Infection and Resistance (1914), pp. 134-217. 



