332 APPENDIX 



C. Staining Values. 



1. Demonstrations of parts of cell. 



2. Identification of species. 



3. Differentiation of species. 



D. Determination of Microorganisms. 



1. Methods employed. 



2. Differential characteristics. 



II. PHYSIOLOGIC MICROBIOLOGY 



A. Cell Studies. 



1. Composition of cell contents. 



2. Composition of cell wall. 



3. Physical products of physiological significance. 



a. Heat. 

 6. Light. 



4. Products of physiological significance of which little is 



known, 

 a. Pigment. 

 6. Enzymes. 



c. Aromatic compounds. 



d. Toxins. 



5. Absorption or assimilation of foreign bodies. 



6. Chemotaxis. 

 5. Phototaxis. 



8. Aerotaxis, 



9. Plasmolysis. 

 10. Plasmoptysis. 



B. Studies in Metabolism. 



1. Elements required in growth of microorganisms. 



2. Respiration. 



3. Nutrition. 



4. Moisture. 



5. Temperature of cultivation. 



6. Conditions of media; reaction, composition, etc. 



