APPENDIX 333 



7. Physiologic test media. 



8. Identification and determination of species of micro- 



organisms by means of 



a. Cultural physiologic methods. 



b. Chemical tests. 



c. Physical tests. 



d. Biological tests. 



9. Enzymes. 



C. Studies in Association. 



1. Symbiosis. 



2. Metabiosis. 



3. Antibiosis. 



D. Common Fermentative Changes Produced by Micro- 



organisms. 



1. Studies in enzymes. 

 a. Formation. 

 Zymogen. 

 Activator. 

 Kinases. 

 6. Kinds. 



c. Actions (specificity) and materials fermented. 



d. Conditions under which enzymes act; 



(1) Physical. 

 Temperature. 



Radiation, light rays (solar, electric, etc.), 

 Rontgen rays, radium rays and emana- 

 tions. 



(2) Chemical and physico-chemical. 

 Activators; kinases, organic acids, bases, 



neutral salts. 

 Protective agents. 

 Paralysors and poisons. 

 Concentration of solutions. 

 Reaction of substrate. 

 Extent of accumulated products. 



