350 APPENDIX 



or several layers of clean cheese-cloth and allow to ferment 

 spontaneously. 



3. Change the water in the bottle occasionally, replacing 

 the amount of water removed, with the same amount of 

 clean, distilled water. 



4. When the active fermentation (as noted by the evolu- 

 tion of gas) has ceased entirely, this agar should be placed 

 in an agateware pail, counterpoised, boiled over a free 

 flame to dissolve the agar, counterpoised and any loss made 

 up with distilled water. 



5. Place in tubes or flasks as desired and autoclav. 



Uschinsky's asparagin medium : protein-free. 



Asparagin, COOH - CH(NH 2 ) CH 2 CO - NH 2 . . 3.4 gms. 



Sodium chloride, NaCl 5.0 gms. 



Magnesium sulphate, MgS04 0.2 gm. 



Calcium chloride, CaCl 2 0.1 gm. 



Monobasic acid potassium phosphate, KH 2 PO4. 1 . gm. 



Iron sulphate, FeSO4 Trace 



Distilled water , , , 1000.0 c.c. 



Cohn's solution: inorganic nitrogen combined with an 

 organic acid. 



Monobasic acid potassium phosphate, KH 2 P04. 5 . gms. 



Calcium phosphate, CasPCU 0.5 gm. 



Magnesium sulphate, MgSCU *. 5.0 gms. 



Ammonium tartrate, CH(OH) COO NH 4 .... 10 . gms. 



CH(OH)-COO-NH 4 

 Distilled water 1000.0 c.c. 



Winogradski's medium for nitrate formation: inorganic 

 nitrogen combined with inorganic acid. 



Ammonium sulphate, (NH4) 2 S04 0.40 gm. 



Magnesium sulphate, MgSO4 . 05 gm. 



Dibasic acid potassium phosphate, K 2 HP04 . . 0.10 gm. 



