374 APPENDIX 



Ziehl-Nielson's carbol-fuchsin. 



Solution A. 



Basic fuchsin 1 gm. 



Absolute alcohol 10 c.c. 



Solution B. 



Carbolic acid 5 gms. 



Distilled water 100 c.c. 



1. Dissolve the fuchsin in the absolute alcohol. (Solu- 

 tion A.) 



2. Dissolve the carbolic acid in the distilled water 

 (Solution B). 



Note. Solutions A and B will keep indefinitely if kept sepa- 

 rate. 



3. Mix in the proportion of 10 c.c. of solution A to 100 

 c.c. of solution B. 



Note. If A and B do not mix readily, warm slightly and add a 

 few drops of absolute alcohol. 



4. Filter. 



Loeffler's alkaline methylen blue. 



Saturated alcoholic solution of methylen blue 30 c.c. 



Potassium hydrate, 0.1% aqueous solution 100 c.c. 



